protein synthesis in rabbit reticulocytes: a radioimmu noassay method for direct determination of eif-2 concen trations in reticulocyte lysates
نویسندگان
چکیده
a radioimmunoassay method for direct determination of eif-2 concentration in reticulocyte lysate has been developed. this assay method measures eif-2 concentratio ns in nanogram range. a value of 40 pmol eif-2 per ml of reticulocyte lysate was obtained.
منابع مشابه
Protein synthesis in rabbit reticulocytes: characteristics of CO-eIF-2 protein complex.
A high molecular weight reticulocyte protein factor, named Co-eIF-2, contains Co-eIF-2A, Co-eIF-2B, and Co-eIF-2C activities and stimulates Met-tRNAf binding to eIF-2 both in the presence and absence of Mg2+. Some characteristics of this stimulation in the absence of Mg2+ are: (1) Stimulation is most pronounced at low eIF-2 levels. (2) Stimulation is partially resistant to heat and NEM treatmen...
متن کاملProtein synthesis in rabbit reticulocytes: a study of the mechanism of action of the protein factor RF that reverses protein synthesis inhibition in heme-deficient reticulocyte lysates.
A eukaryotic initiation factor 2 (eIF-2)-ancillary protein factor Co-eIF-2 promotes displacement of GDP from eIF-2 X GDP and facilitates ternary complex (Met-tRNAf X eIF-2 X GTP) formation in the presence of Mg2+. Heme-regulated protein synthesis inhibitor, HRI, phosphorylates the alpha-subunit of eIF-2 and thus inhibits ternary complex formation as Co-eIF-2 does not displace GDP from eIF-2 alp...
متن کاملPurification and characterization of protein synthesis initiation factors eIF-1, eIF-4C, eIF-4D, and eIF-5 from rabbit reticulocytes.
Protein synthesis initiation factors eIF-1, eIF-4C, eIF4D, and eIF-5 were isolated and purified from rabbit reticulocyte lysates. Crude initiation factors from the high salt ribosomal extract were separated by ammonium sulfate precipitation into Fraction A (0 to 40% saturation) and Fraction BC (40 to 70% saturation). Fraction BC was separated by gel filtration on Sephadex G-100 into two fractio...
متن کاملProtein synthesis in rabbit reticulocytes: characteristics of the protein factor RF that reverses inhibition of protein synthesis in heme-deficient reticulocyte lysates.
During heme deficiency in reticulocyte lysates, the heme-regulated translational inhibitor of protein synthesis (HRI) is activated and shuts off protein synthesis. In partial reactions, HRI phosphorylates the Mr 38,000 subunit (alpha subunit) of eukaryotic initiation factor 2 (eIF-2), which forms a ternary complex, Met-tRNAf X eIF-2 X GTP. The eIF-2 alpha (P) thus formed is not recognized by tw...
متن کاملCharacterization of a rat liver factor that inhibits initiation of protein synthesis in rabbit reticulocyte lysates.
Protein synthesis in rabbit reticulocytes and their lysates is regulated by heme. In heme-deficient reticulocyte lysates, protein synthesis proceeds at the initial rate for several minutes and then declines abruptly. Inhibition of protein synthesis is due to the activation of a heme-regulated translational inhibitor (HRI) which blocks the initiation of protein synthesis. Addition of the isolate...
متن کاملProtein synthesis in rabbit reticulocytes: characteristics of a ribosomal factor that reverses inhibition of protein synthesis in heme-deficient lysates.
A ribosomal salt (0.5 M KCl) wash factor (RF) that reverses inhibition of protein synthesis in heme-deficient reticulocyte lysates has been resolved from the bulk of Met-tRNAfMet-binding factor (EIF-1), Co-EIF-1, and EIF-2 (ternary complex dissociation factor, TDF). The purified RF restores protein synthesis activity of heme-deficient lysates to the level observed in the presence of hemin. No d...
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عنوان ژورنال:
acta medica iranicaجلد ۲۷، شماره ۱-۴، صفحات ۱-۱۳
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